Система Orphus



December №4 (18) 2016

Автор и название статьи

Аннотация и ключевые слова

A.G. Bliummer, Researcher of Voronezh branch of FGBU “VNIIKR”


UDC 632.725.6


The Psyllid Acizzia jamatonica Kuwayama, 1908 (Hemiptera: Psyllidae: Acizzinae), a Dangerous Pest of Albizia from the Eastern Asia, Introduced into the Crimea and the Krasnodar Region


Abstract. The present article touches upon the history of spreading of Acizzia jamatonica Kuwayama, a dangerous pest feeding on Albizia julibrissin, beyond its natural habitat and forming its secondary (invasive) area. The article includes data concerning the psyllid’s spreading in Crimea and in the Krasnodar region. The text provides characteristics of its biology, harmfulness, and chemical control measures. The work mentions the physllid’s entomophags detected in its colonies on Albizia in Crimea, and suggests the perspectives of using a biomethod to control the phytophag’s populations. The article suggests giving the phytophag the status of a regulated non-quarantine pest in the future.

Keywords. Acizzia jamatonica, Psyllidae, Acizzinae, phytophag, Albizia julibrissin, introduction, Europe, Crimea, the Krasnodar region.

Y.N. Kovalenko, Researcher of Scientific and Methodological Department of FGBU “VNIIKR”


UDC 591.9


The Entomologic Expedition to the Republic of Peru

Abstract. This article describes the basic data about the entomological expedition to the Republic of Peru, carried out by FBGU “VNIIKR” experts in April 2016.  The expedition route and the main points of entomological collections and permanent scientific work have been also considered on this topic. It also provides an overview of the material, collected during the trip as well as obtained following to the scientific exchange (including by species having the quarantine importance for the Russian Federation).

Keywords. Peru, Expedition, Quarantine object, Plant pests, Insects, Insecta.


O.A. Kulinich,  Head of Forest Quarantine Department FBGU «VNIIKR»


UDC 632.6.04+58.073


Bacteria Associated with Wood-Inhabiting Nematodes of Bursaphelenchus, as Potential Causative Agents of Pine Wilt Disease

Abstract. The 20 bacterial species belonging to 13 genera have been isolated and identified by 16S rRNA gene sequencing from 25 nematode isolates of Bursaphelenchus mucronatus collected from different regions of the Russian Federation (RF). The most common bacteria were species of Pseudomonas spp. (frequency of occurrence 96%), followed by Stenotrophomonas (64%), Pantoea (28%), Bacillius (8%), Burkholderia (4%), and Serratia (4%). The occurrence of Pseudomonas brenneri and P. fluorescens bacteria was 52% and 36%, respectively. Also bacterial species of two Portuguese and one American B. xylophilus nematodes isolates has been studied. Ten associated bacteria species have been identified: Agrobacterium tumefaciens, Pseudomonas fluorescens, P. brenneri, Rahnella aquatilis, Stenotrophomonas maltophilia, S. rhizophila, Yersinia mollaretii. The pathogenicity of bacteria strains was determined by hypersensitive reaction to Nicotiana tabacum L. tobacco leaves. Among 50 strains of 16 bacteria species, necroses have been marked only for Pseudomonas brenneri and P. fluorescens. This pathogenic strain of P. fluorescens was extracted from B. xylophilus nematode, and two strains of Pseudomonas brenneri and P. fluorescens – from Russian isolates of B. mucronatus. Out of 25 Russian nematode isolates of B. mucronatus only two ones contained bacteria strains of Pseudomonas brenneri and P. fluorescens, which induced necrosis.

Keywords. Pine wilt disease, nematodes, symbiotic bacteria, pathogenicity, Bursaphelenchus mucronatus, Bursaphelenchus xylophilus.


A.V. Filippov, Leading Agronomist in Buryat Research Laboratory of FGBU “VNIIKR” Irkutsk Branch


UDC 591.91


Entomology Expedition to Kyrgyzstan to the Inner Tian Shan


Abstract. The entomological expeditions to Kyrgyzstan and to the Inner Tian Shan have been realized. Due to results of carried out researches the faunal list has been compiled and the various insect orders have been gathered to replenish entomological collection.

Keywords. Biodiversity, entomofauna, lepidopterology, Kyrgyzstan, the Inner Tian Shan.


N.M. Atanov, Leading Researcher of FGBU “VNIIKR”


UDC 632.7.08


Impact of Modifiers on the Attractiveness of the Horse-Chestnut Leaf-Miner’s Pheromone Cameraria ohridella Deschka et Dimi?, 1986

Abstract. The object of the research is the horse-chestnut leaf-miner moth Cameraria ohridella. The impact of two modifiers and their combination with a standard dose of pheromone on the attractiveness in “Delta” adhesive trap has been studied.

As a result of the researches “Delta” adhesive trap with the dispenser (rubber stopper) containing the pheromone in the amount of 2 mg + the modifier can be recommended.

Keywords. Horse-chestnut leaf-miner moth, Cameraria ohridella, pheromone, dispenser, traps, preparative form, attractiveness, modifier.


G.N. Matyashova, Researcher, Research and Testing Department of Scientific and Analytical Center of FGBU “VNIIKR”


UDC 632.911.2/632.913.1


Training for FGBU “VNIIKR” Experts in the Field of Diagnosis of Plant Pathogens at the National Institute of Biology in Slovenia

Abstract. The article provides the results of the training of FGBU “VNIIKR” experts in the Republic of Slovenia. During the professional training on the study and diagnosis of viruses, viroids and pathogenic phytoplasmas of crops diseases such methods as the real-time PCR, the LAMP-PCR, the submicroscopy, the new-generation sequencing (NGS) were considered as well as the automation of laboratory examination of phytopathogens and of viral particle concentration in order to identify them in a closed-loop irrigation.

Keywords. Diagnostics, viruses, viroids, phytoplasmas, PCR, international cooperation.